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. Block diagram of an HPLC–MS. A 3 component mixture enters the HPLC. When ingredient A elutes in the column, it enters the MS ion source and ionizes to sort the mother or father ion and a number of other fragment ions.. HPLC separation of a mix of flavonoids with UV/Vis detection at 360 nm and, while in the inset, at 260 nm. The choice of wavele